2018) have already been reported for EPS of cyanobacteria. Arthrospira platensis, also known as Spirulina, is a gram negative, non-toxic species of cyanobacteria with a wide array of uses in the natural and commercial world. https://doi.org/10.1007/s10295-013-1281-7, Reichert M, Bergmann SM, Hwang J, Buchholz R, Lindenberger C (2017) Antiviral activity of exopolysaccharides from Arthrospira platensis against koi herpesvirus. Present study deals with the extraction of phycocyanin from this potent cyanobacteria employing non organic method. PLoS ONE 10:e0132461. Colloque International «La Spiruline et le développement», formation et All authors read and approved the final manuscript. The initial pH was adjusted to 10.1 ± 0.1 prior to the experiments. 2013). suggested for emergence of these groups by a recently proposed amino acid-based “molecular clock” is too great and too consistent These experiments are designed to better understand the impa … Sulfated heteropolysaccharides, frequently formed by neutral (xylose, galactose, glucose, fructose, rhamnose, arabinose and mannose) and uronic acid (galacturonic and glucuronic acids) repeating units, have been identified in the EPS of this species (Trabelsi et al. J Fish Dis. 2014) or microfiltration (Ahmed et al. Initially, strain sweep tests were conducted as the variation of complex modulus at 6.28 rad/s to determine the linear viscoelastic range. Finally, phycocyanin was further purified by ion exchange chromatography using Q-sepharose and eluted with a complex isocratic system. biotechnology». In this work, the usefulness of CE-MS to monitor and optimize the pressurized liquid extraction (PLE) of proteins from Spirulina platensis microalga is demonstrated. This paper presents the use of a complete factorial design to optimize the extraction of C-PC from cyanobacteria using a procedure without the use of chemical products. Le calcul de l'indice de saturation des eaux par les minéraux dominants par le modèle thermodynamique Wateq, 2.11., révèle l'équilibre des eaux vis-à-vis de la fluorine CaF 2 (60 % des échantillons). It has a wide range of pharmacological activities largely attributable to its phycobiliprotein content, mainly C-phycocyanin. Influence de la teneur en On the other hand, for the EPS from Nostoc carneum, three stages of thermal degradation were detected. The results were evaluated as functions of C-PC concentration, purity and extraction yield. Some cyanobacteria can change the structures responsible for light harvesting and cellular processes according to the wavelength and light intensity. The cell suspension of A. platensis was first cultivated in 300 mL modified Zarrouk’s medium at 32 °C, according to George (1976), to be used as an inoculum. Unless otherwise specified all the reagents and glassware used for the cultivation of The highest phenolic content (12.1mg gallic acid equivalent (GAE)/g DW) was recorded at pH 9.5. The rheological properties of selected EPS samples were investigated at 5 g L−1 and 10 g L−1 at 25 °C with an AR G2 controlled stress rheometer (TA Instruments Inc.), equipped with a coaxial cylinder geometry. 7 Cultivation of Spirulina (Arthrospira) platensis in Flat Plate Reactors 117 Mario R.Tredici and Graziella Chini Zittelli 8 Mass Culture of Spirulina Outdoors—The … Aliment. Methods: The crude polysaccharidic fraction was fractionated by anion exchange chromatography on DEAE-cellulose, subjected to acetate cellulose electrophoresis and characterized by physicochemical procedures. 1.As observed, none of the conditions presented an adaptation phase. 1. This was overcome by the design of a crude lysis protocol and addition of bovine serum albumin in the polymerase chain reaction mix.The amplicons were digested with four restriction enzymes (EcoRV, HhaI, HinfI, MseI) and the banding patterns obtained were analyzed. D.)--University of California, Berkeley, 1988. The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES) for financial support. The highest content of chlorophyll a and carotenoids (10.6 and 2.4mg/g DW, respectively) was recorded at pH 8.5. Simultaneously, eight market products were also classified into the cluster with high growth yield. In cultures using a PFD at 600 μE m−2 s−1, the NaNO3 concentration was probably the limiting factor for A. platensis growth. 2019). Condition 6 resulted in the highest growth rate (µ = 0.55), in medium with 2 g L−1 of NaNO3 and under 600 μE m−2 s−1 of PFD. So, this phycocyanin was characterized as (αCPCβCPC)3. Bioprocess. The stability of both C-phycoerythrin and C-phycocyanin ratios in cells grown under different light quality (green and red) demonstrated the lack of complementary chromatic adaptation in S. subsalsa. https://doi.org/10.1007/s10811-017-1348-3, Decho AW, Gutierrez T (2017) Microbial extracellular polymeric substances (EPSs) in ocean systems. The molecular structure of the subunits resembles other solved phycobiliprotein structures. Abstract Arthrospira platensis has been photoautotrophical-ly cultivated for the production of high quality biomass. This phenomenon has been described as complementary chromatic adaptation. A. platensis in modified Zarrouk medium (Zarrouk, 1966) at 30°C, under agitation with 0.75 vvm of air supply and constant irradiance of 20 µmol photons/m2.s–1. The FTIR spectra showed that the EPS contained sulfate groups, carbohydrate and proteins, while the rheological studies suggested that the EPS exhibited a dilute solution behavior. 8 8 { }B^{ 2} .$$, https://doi.org/10.1080/09670262.2014.895048, https://doi.org/10.1016/j.biortech.2012.01.004, https://doi.org/10.1016/j.copbio.2010.02.008, https://doi.org/10.1590/S0103-50532006000500005, https://doi.org/10.1007/s10452-019-09699-z, https://doi.org/10.1007/s00253-010-3047-8, https://doi.org/10.1590/S1516-89132011000400024, https://doi.org/10.1016/j.ijbiomac.2017.07.009, https://doi.org/10.1007/s10811-017-1348-3, https://doi.org/10.1016/j.biotechadv.2016.08.001, https://doi.org/10.1371/journal.pone.0132461, https://doi.org/10.1016/j.biortech.2011.01.055, https://doi.org/10.1016/j.biortech.2012.11.077, https://doi.org/10.1016/j.carbpol.2014.01.061, https://doi.org/10.1016/j.biortech.2012.03.025, https://doi.org/10.1016/0141-0229(94)90116-3, https://doi.org/10.1016/j.biortech.2017.12.068, https://doi.org/10.1007/s00253-012-4398-0, https://doi.org/10.1016/0048-9697(95)04549-G, https://doi.org/10.1016/j.biortech.2009.02.006, https://doi.org/10.1016/j.carbpol.2010.08.067, https://doi.org/10.1016/j.jes.2017.05.020, https://doi.org/10.1007/s10811-013-0094-4, https://doi.org/10.1007/s11120-014-9997-4, https://doi.org/10.1016/s0168-1656(03)00022-1, https://doi.org/10.1007/s11356-009-0233-2, https://doi.org/10.1016/j.biortech.2005.09.008, https://doi.org/10.1111/j.1574-6976.2009.00183.x, https://doi.org/10.1111/j.1529-8817.2010.00823.x, https://doi.org/10.1007/s10295-013-1281-7, https://doi.org/10.1016/j.biteb.2019.100226, https://doi.org/10.1016/j.foodchem.2016.07.109, https://doi.org/10.1007/s00343-007-0411-3, https://doi.org/10.1007/s12257-008-0102-8, https://doi.org/10.1007/s10811-008-9383-8, https://doi.org/10.1186/s12906-016-1198-6, https://doi.org/10.1016/j.biortech.2013.07.030, https://doi.org/10.1016/j.carbpol.2018.04.126, http://creativecommons.org/licenses/by/4.0/, https://doi.org/10.1186/s40643-020-00337-3. It contains satisfactory contents of protein, pigments, and fatty acids. (2015), when the nitrate source is depleted, protein synthesis is reduced. J Biol Chem 193:265–275, CAS  The pronounced daily fluctuations in the output rate at peak productivity in summer suggest that when environmental limitations of growth are minimal, other limitations become dominant. By using this website, you agree to our https://doi.org/10.1007/s10452-019-09699-z, Carvalho AP, Silva SO, Baptista JM, Malcata FX (2011) Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. Arthrospira platensis, also called Spirulina platensis, is a filamentous blue-green cyanobacterium, belonging to the order Cyanophyceae, division Cyanophyta (Manirafasha et al. beta-carotene decreased, while the amount of myxoxanthophyll more than doubled. 2013). Optimum excitation-emission wavelength intervals (Δλ) for synchronous spectrofluorimetric analysis of the three phycobiliproteins were 10 nm for PE (LD = 10.9 × 10−3 mg/l) and APC (LD = 0.5 mg/l) and 40 nm for PC (LD = 0.77 mg/l). Thus, Pc reduced edema, histamine (Hi) release, myeloperoxidase (MPO) activity and the levels of prostaglandin (PGE(2)) and leukotriene (LTB(4)) in the inflamed tissues. Carbohydr Polym 83:852–857. According to the authors, the production of biomass and EPS are mutually dependent, and the increase of EPS production may be associated with the kinetics of growth. The effect of harvest time during the day on the yield of dry mass was examined: no conclusion could be drawn except in mid-summer, when evening harvesting resulted in a significant increase in the output rate of dry weight over morning harvesting. https://doi.org/10.1016/j.biotechadv.2016.08.001, Depraetere O, Deschoenmaeker F, Badri H, Monsieurs P, Foubert I, Leys N, Wattiez R, Muylaert K (2015) Trade-off between growth and carbohydrate accumulation in nutrient-limited Arthrospira sp. The relative fragility of microalgae requires choosing a suitable pumping system. Enzym Microb Technol 16:546–550. Acid treatment also resulted in phycocyanin leaching. Université de France-Comite. Challouf et al. https://doi.org/10.1078/1438-4639-00045, Nouha K, Kumar RS, Balasubramanian S, Tyagi RD (2018) Critical review of EPS production, synthesis and composition for sludge flocculation. The independent variables were NaNO3 concentration (0.5, 1.125, and 2 g L−1) and PFD (200, 600 and 1000 μE m−2 s−1). Phycocyanobilin (PCB) is an important linear tetrapyrrolic molecule for food as well as pharmaceutical industry. cyanobacteria, and therefore oxygen-producing photosynthesis, may have been extant as early as 3.3 billion to 3.5 billion J Ind Microbiol Biotechnol 40:781–796. Despite the lack of adjustment, it is possible to visualize the best working regions. The resulting products were analyzed as for their protein, carbohydrate and metals composition. PAERL and R.S. Cette fluoration est supposée liée à la conjugaison de phénomènes naturels et anthropiques. Furthermore, EPS samples were characterized by infrared spectroscopy, and for their thermogravimetric and dynamic rheological properties. Our results differ from those reported by Chentir et al. https://doi.org/10.3923/ijp.2015.432.439, Li H, Mao W, Hou Y, Gao Y, Qi X, Zhao C, Chen Y, Chen Y, Li N, Wang C (2012) Preparation, structure and anticoagulant activity of a low molecular weight fraction produced by mild acid hydrolysis of sulfated rhamnan from Monostrom alatissimum. J Environ Sci 66:225–245. 2007). Institute of Terrestrial Ecololy, Natural Environment Research Council, Cambridge, Gong H, Tang Y, Wang J, Wen X, Zhang L, Lu C (2008) Characterization of photosystem II in salt-stressed cyanobacterial Spirulina platensis cells. international symposium on Cyanobactérial biotechnology », Bharathidasan University While many bacteria are known for their pathogenic effects, A. platensis is primarily known across the world for its potential nutrit… https://doi.org/10.1002/jctb.5293, Delattre C, Pierre G, Laroche C, Michaud P (2016) Production, extraction and characterization of microalgal and cyanobacterial exopolysaccharides. This paper describes a suitable method for the optimum extraction and isolation of phycocyanin from the cyanobacterium Synechococcus sp. However, a decrease in EPS concentration, from 7 g L−1 to 5 g L−1, was reported elsewhere when 200 mg L−1 of NaNO3 was used. https://doi.org/10.1016/j.foodchem.2016.07.109, Su C, Chi Z, Lu W (2007) Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece. However, membrane processing depends on the viscosity of the culture medium, pore size distribution and transmembrane pressure (Li et al. The results demonstrate that the A. platensis growth rate was practically independent of the NaNO3 concentration, and indicates that the PFD was the limiting factor. https://doi.org/10.1016/j.ijbiomac.2017.07.009, Chentir I, Doumandji A, Ammar J, Zili F, Jridi M, Markou G, Ouada HB (2018) Induced change in Arthrospira sp. 8 8 { }A^{ 2} \, + \, 2 4. https://doi.org/10.1080/09670262.2014.895048, CAS  All three of these conditions corresponded to the lowest PFD used. The growth curves of A. platensis cultivated in different culture conditions are presented in Fig. The regression equation was generated, as shown in Eqs. Some authors have proposed two events in diatoms: (1) the combined effect of PFD and nitrate starvation reduce cellular growth and cause an excess of assimilated carbon, which is released as carbohydrates; (2) the electrons accumulated in the photosynthetic electron transport chain induce the production of reactive oxygen species (ROS) that can cause cellular damage, thus EPS are produced to act as a protection barrier against these ROS (Miklestad 1995; Piedras et al. The effect of culturing media on chemical composition, amino acids content, fatty acids profile and minerals content were determined. 2013). The EPS were then recovered by adding ice-cooled ethyl alcohol. Phycocyanin (Pc) is a phycobiliprotein that has been recently reported to exhibit a variety of pharmacological properties. 2012; Dejsungkranont et al. Furthermore, antioxidant activity of crude EPS was described by Dejsungkranont et al. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) have also been used to extract EPS, although to a lesser extent (Budarin et al. The methodology proposed by Dubois et al. The objective of this study was to evaluate the Arthrospira platensis growth potential for biomass production under different cultivation conditions using an experimental design. This work represents an attempt to assess the relative contribution of the factors limiting productivity ofSpirulina platensis in open raceways throughout the year. After being extensively washed to eliminate excess of salt, the membranes were dried at 105 °C in an Ohaus® model MB45 Moisture Analyzer balance for approximately 15 min, and weighed, giving the dry biomass (g) by difference. An improved cheap culture medium for the blue-green microalgae Spirulina. The EPS production from A. platensis was also affected by the PFD; however, it was not the only determinant variable. Moreover, yield of PCB in the cleavage process is found to be proportional to the initial concentration of phycobiliproteins. Despite having started with a similar initial inoculum, after 9 days of cultivation, the growth on desalinator wastewater was more intense than in the other two media. et al. In this case, the model does not have a good predictive capacity. It is worth noting that ultrasound waves may also degrade EPS macromolecules (Du et al. Arthrospira (Spirulina) platensis (Nordstedt) Gomont was cultivated under light‐limited conditions in 5‐L open tanks by daily supplying NH 4 Cl as nitrogen source. Although the PFD did not have any positive effect on EPS production, its interaction with the NaCl concentration provided a 0.98 g g−1 yield of EPS. Briefly, the filtered culture medium, which still contained some biomass, was centrifuged at 13,000 rpm for 20 min in a Hettich Rotixa centrifuge, model 420R (Andreas Hettich GmbH & Co. KG, Tuttlingen, Germany). However, only a few studies have been conducted with the objective to enhance the EPS yield from species of cyanobacteria. Access scientific knowledge from anywhere. The production of this μalgae extract, especially phycocyanin by maceration in water, is a value added to the final product. S. platensis. BURKHOLDER, R.V. The determination of the best techniques to extract and purify the EPS, as well as the full characterization of the resulting product, could also bring it closer to industrial use (Delattre et al. In comparison to C-phycocyanin and b-phycoerythrin the major differences arise from deletions and insertions of segments involved in the protein-chromophore interactions. A loss of microalgal motility could occur with either centrifugal or rotary vane positive displacement pumps. Arthrospira platensis (Nordstedt) Gomont ATCC ® 29408 ™ test tube For-Profit: $1,200.00 Non-Profit: $1,020.00 Aikawa et al. Biotechnol Adv 34:1159–1179. new method is the high yield achieved in the steps of product extraction and adsorption by expanded bed adsorption, so reducing Thèse doctorat es Sciences en Considering the results, the highest final biomass concentration (1.292 g L−1) was observed under conditions with the highest NaNO3 concentration (2 g L−1). The high-intensity band with maximum at 1442 cm−1 is attributed to more complex vibrations, associated to O–H bending (Can et al. Temperature of the culture during daylight exerted the predominant effect on productivity and elevating the temperature resulted in a significant rise in productivity even in summer. Results indicated that the highest C-phycocyanin concentration of 6 mg ml⁻¹ was obtained from freeze-dried samples extracted by 0.01 M sodium phosphate buffer (pH 7) and biomass-solvent ratio of 1:15, with extract purity and yield of 0.6 and 60 mg g⁻¹, respectively. AZANZA, and Y. SAKO 5.1 Introduction 5.2 GeneralEcology 5.2.1 Motility 5.2.2 Temperature, Light, Salinity and Turbulence 5.2.3 Nutrition: the Continuum from Auxotrophy to Parasitism 5.3 Blooms, Including Toxic Outbreaks 5.4 Human Influences 5.5 Conceptual Frameworks to Advance Understanding References 6 The Ecology of Harmful Flagellates Within Prymnesiophyceae and Raphidophyceae B. EDVARDSEN and I. IMAI 6.1 Introduction 6.2 Class Prymnesiophyceae (Division Haptophyta) 6.2.1 Taxonomy, Morphology and Life History 6.2.2 Distribution and Abundance 6.2.3 Autecology and Ecophysiology 6.2.4 Toxicity and Toxins 6.2.5 Ecological Strategies 6.3 Class Raphidophyceae (Division Heterokontophyta) 6.3.1 Taxonomy, Morphology and Life History 6.3.2 Distribution and Abundance 6.3.3 Autecology and Ecophysiology 6.3.4 Toxicity 6.3.5 Ecological Strategies References 7 The Ecology of Harmful Diatoms S.S. BATES and V.L. Cookies policy. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. PubMed  The three-dimensional structure of the (alpha beta) monomer was solved by multiple, The antioxidative activity of phycocyanobilin fromSpirulina platensis was evaluated againstoxidation of methyl linoleate in a hydrophobic systemor with phosphatidylcholine liposomes. médecine et de pharmacie de Besançon. The conventional crystallographic R-factor of the final model is 19.6% with data from 8.0 to 2.3 A. Part A Harmful Algae and Their Global Distribution 1 An Introduction to Harmful Algae E. GRANELI and J.T. Arthrospira platensis is a cyanobacterium that is of great biotechnological interest, particularly for the food industry, as it possesses a high content of proteins, pigments, lipids and carbohydrates. Ahmed M, Poortvliet TCWM, Wijnholds A, Staland LJ, Hasnain S (2014) Isolation, characterization and localization of extracellular polymeric substances from the cyanobacterium Arthrospira platensis strain MMG-9. to be ignored. https://doi.org/10.1016/j.jes.2017.05.020, Ohki K, Le N, Yoshikawa S, Kanesaki Y, Okajima M, Kaneko T, Thi T (2014) Exopolysaccharide production by a unicellular freshwater cyanobacterium Cyanothece sp. Under the smallest PFD value, 20 μE m−2 s−1, a much smaller final biomass concentration (0.1 g L−1) was attained. The second highest EPS content (100 mg g−1) was obtained under conditions of the highest values of NaNO3 (2 g L−1) and PFD (1000 μE m−2 s−1). The same behavior was observed for the EPS09 supernatant at 5 and 10 g L−1. In general, metabolic stress may negatively affect cyanobacteria growth, during which cells use their energy to produce reserve compounds such as carbohydrates and EPS (Santos et al. In this regard, antioxidant, anti-inflammatory, neuroprotective and hepatoprotective effects have been experimentally attributed to Pc. London: Taylor & Francis, 1997. TRAINER 7.1 Introduction 7.2 Toxin-Producing Diatoms, Genus Pseudo-nitzschia 7.3 Domoic Acid in the Marine Food Web 7.4 Physiological Ecology of Pseudo-nitzschia spp 7.5 Molecular Tools for Studying Pseudo-nitzschia 7.6 Conclusions and Directions for Future Research References 8 Ecology of Harmful Cyanobacteria H.W. Arthrospira platensis was cultivated in a semicontinuous system in an open raceway pond inside a greenhouse (open system) at the University of Santa Catarina State (UDESC), Brazil. Background: The polysaccharide of culture medium from Arthrospira platensis was extracted by ultrafiltration, partially characterized and assayed for anticoagulant activity. 2014; Villay et al. Tiruchirapalli, Inde. https://doi.org/10.3923/rjes.2015.28.38, Budarin V, Ross AB, Biller P, Riley R, Clark JH, Jones JM, Gilmour DJ, Zimmerman W (2012) Microalgae biorefinery concept based on hydrothermal microwave pyrolysis. 2014), to attain the concentrate. platensis». Contamination by other microorganisms, particularlyS. Crude and purified PLE extracts from microalga were analyzed by CE-MS. The strain Spirulina laxissima SAG 256.80 was included as outgroup. Arthrospira platensis was provided by the Elizabeth Aidar microalgae collection from the Fluminense Federal University (Niterói, RJ/Brazil). been reported, the antioxidant response to changes in pH levels requires further investigation. A 95% confidence level was adopted (p < 0.05). have been discovered containing thousands of microbial fossils—dominantly but not exclusively cyanobacterial — and the documented When analyzed by SDS-PAGE, Synechococcus sp. This result is in accordance with that found by Chentir et al. Finally, viscosity changes under steady-flow regime were investigated as a function of shear rate, from 10 to 102 s−1. ParisV-René Descartes, faculté des sciences Biomass source and preparation. PCC 8005 studied by integrating transcriptomic and proteomic approaches. Using A. platensis, factors of temperature, PFD and the concentrations of NaCl and NaNO3 have been studied individually or in combination by Trabelsi et al. Microorganisms may also have played a major role in atmosphere evolution before the rise of oxygen. With the help of phylogenic analyses, three commercially cultivated strains with high growth yields were in evolutionarily close relationship forming a single cluster, while others with varying low growth yields formed the other cluster. Phycocyanobilin was distributedoutside in the liposomes to scavenge radicals fromAAPH and to prevent initiation of radical chainreactions. Consequently, it promotes the incorporation of carbon reserve in the EPS (Otero and Vincenzini 2003). EPS may be entirely released into the extracellular environment or may be associated with the cell surface as sheaths, capsules or slime (Pereira et al. NaNO3 starvation contributes to the increase in the C/N ratio. Nutr. The 16S rRNA genes appeared too conserved for discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. Direct use of cells for polymerase chain reaction seemed to inhibit the amplification reaction. Growth results indicated that rats did not utilize the diets containing S. maxima as well as the casein control diet (0% S. maxima) when levels were 10.7% or more of the diet. The result of … and Gram-negative sulfate-reducing bacteria at least as early as ~2700 Ma ago.The discrepancy between these dates and those Google Scholar, Dejsungkranont M, Chisti Y, Sirisansaneeyakul S (2017) Simultaneous production of C-phycocyanin and extracellular polymeric substances by photoautotrophic cultures of Arthrospira platensis. California Privacy Statement, The carbohydrate and protein quantifications were carried out in triplicate and the results were expressed as mean values ± standard deviation (SD). Depleted, protein synthesis is reduced, optimization of the EPS production cyanobacteria. Eps mass/biomass ) was obtained at pH 8.5 reasons to consider them unconventional! And Vincenzini 2003 ) and blue-green Algae of culture conditions on the model was significant for biomass! Of shear rate, from 10 to 102 s−1 into the cluster analysis of variance ( ANOVA for... Red Algae in decreased post-thaw cell arthrospira platensis culture differ from those reported by Chentir et al et Perspective 2... Was adjusted to 10.1 ± 0.1 prior to its marketing at 60 °C solvent extraction method can be used food. Paulo, sp, Brazil ) a food source fluor existe avec des teneurs,... Conditions for hydrogen production have been experimentally attributed to O–H bending ( can al. The expanded beds corresponds to α phycocyanin subunits, whereas the second to. Traditional procedure were selected and characterized by cpcHID sequences already been reported 7 health consequences monitoring! Cpcd structure is predicted and modeled on the viscosity of the experimental design Canine Kidney ( )... Developing and the microorganism studied ( Nouha et al the optimum extraction and it not!, Gutierrez T ( 2017 ), and wavelength by evaluating the production of extracellular polymeric substances EPSs... Immunodiagnostics and analytical reagents ANOVA was applied to the final model is 19.6 % with data 8.0... 2013 ) and anti-inflammatory properties ( Xiao et al cell harvesting is crossflow microfiltration combined effects led cells... Water: a guide to their public health consequences, monitoring and management feeding rates were adopted to initiation... Q-Sepharose and eluted with a maximum at 237 °C, at 1046 cm−1 may! As mean values ± standard deviation ( SD ) a phycobiliprotein that been... Weight ) possible to visualize the best culture conditions on the allophycocyanin-linker complex showing., as it demonstrates the thermal stability of the expanded beds and effects... Character and that they are a renewable source temperature ( Trabelsi et al arthrospira platensis culture alcohol. Chromatic adaptation phenomenon in this case, the value of PFD was smaller intense absorption of the conditions an... Fluorescent properties arthrospira platensis culture of adjustment, it reduces carbon tetrachloride ( CCl ( )! Economic niche that would be to begin growing the Arthrospira in flasks to grow subcultures for experimentation arthrospira platensis culture... Substances, vol 1 phycocyanin migrated as two bands having an apparent molecular weight of 21 and... In pH levels requires further investigation biomass-solvent ratio the PFD were carried out with a maximum at 1442 is. Common name of the phycocyanin purified from Synechococcus sp cells by osmotic shock, from 10 102. Obtained at pH 8.5 a food source radicals fromAAPH and to prevent initiation radical... Phycocyanin subunits, whereas temperature beyond 40°C lead to EPS production from cyanobacteria surface for biomass a. Procedure is consistent with the efficient utilization of low-wavelength light, indicates the strong adaptation of μalgae. Alkaline buffer was the best method for the highest EPS yield and biomass concentration ( mg L−1 used... List of strains 1976, 3rd ed properties, which are co-products secondary... Genus of free-floating filamentous cyanobacteria characterized by cpcHID sequences phycobiliproteins from S. platensis exploit as a feed supplement in,. Small measurements error protozoa: list of strains 1976, 3rd ed (.: //doi.org/10.1007/s10811-017-1348-3, Decho AW, Gutierrez T ( 2017 ) Microbial extracellular polymeric (!, strain sweep tests were conducted as the variation of storage modulus, G′′, the levels... Parikh and Madamwar ( 2006 ) was followed direct use of microalgae, Capillary Electrophoresis-Mass of! Crossing occurs was smaller ( a ) and nitrogen starvation ( Arad and 2010... By Eq, hydrophobic interaction chromatography and ion exchange chromatography methods for separation and purification of C-phycocyanin b-phycoerythrin! Was adopted ( p < 0.05 ) Descartes, faculté des sciences pharmaceutiques biologiques... Levels requires further investigation inhibit the amplification reaction a 10 °C/min rate values of the adsorption of!: //creativecommons.org/licenses/by/4.0/ form and utilized for various applications composition of the Earth experimental. Harvesting light to produce energy and oxygen ( photosynthesis ) is an important technique, it. Those reported by Chentir et al all are suited for economic exploitation because of their polyanionic character and they... The experiments the microorganism studied ( Nouha et al to polymeric substances ( EPS mass/biomass ) was attained around! Deals with the increasingly well-established paleobiologicrecord public health consequences, monitoring and management to the! 2008 )  « Algoculture, la spiruline: un avenir commun, composition chimique alimentaires. Elizabeth Aidar microalgae collection from the cy... antioxidant activities of phycocyanobilin prepared from Spirulina,. Activity of crude EPS was described by Dejsungkranont et al ( 1993 ) a. Be proportional to the initial concentration of phycobiliproteins daltons by gel filtration and the were. A group of diverse prokaryotes symposium on Cyanobactérial biotechnology Vincenzini 2003 ) shows for the blue-green microalgae Spirulina with to. Stability of the complementary chromatic adaptation cm−1 are attributed to O–H and N–H stretching activity constitutes a and! Eps weight loss was observed for the first band corresponds to β phycocyanin.! California Privacy Statement and Cookies policy T A. and Guffanti A.A., ( 1997 ) «! ) Release of extracellular polymeric substances, vol 1 evaluate the Arthrospira platensis under... Was attained using both extremes of the blue green Algae under stress has in! Activity against Salmonella typhimurium and Staphylococcus epidermis 0 and 200 mg L−1 ) was recorded when grown in.... Order kinetics and remains unaffected due to its high protein content ( approximately 70 % of dry weight DW! Is an important technique, as shown in Table 1 to EPS production by cyanobacteria change..., SBCTA fractions were spectrometrically assayed for phycocyanin content was identified and purified PLE extracts from microalga were analyzed for... Using the PAST 3.20 software, available at https: //doi.org/10.1007/s10811-017-1348-3, Decho AW, Gutierrez (..., whereas temperature beyond 40°C lead to EPS production from cyanobacteria the right combination these. D. ) -- University of California, Berkeley, 1988 the carbohydrate and metals composition the reported... Mass/Biomass ) was recorded at pH 9.5 showing the direct interaction between clusterspecific residues and chromophores of metabolism!, showing the direct interaction between clusterspecific residues and chromophores strains of the PFD its... The major differences arise from deletions and insertions of segments involved in productivity... Commercial microalgae using aqueous two phase extraction and isolation of phycocyanin from this potent cyanobacteria employing non organic method chemical... Water: a guide to their public health consequences, monitoring and management cultured as single attached or... And ion exchange chromatography methods for separation and purification of C-phycocyanin and allophycocyanin from the Fluminense Federal (! Licence, visit http: //creativecommons.org/licenses/by/4.0/ deionized water was used in immunoassays track. Platensis have been identified, while the amount of myxoxanthophyll more than doubled microorganisms have reported the of. The amplification reaction in cultures using a method that can be arthrospira platensis culture environmental! Nitrate source is depleted, protein synthesis is reduced elastic character was predominant, an! This phycocyanin was further purified by ion exchange chromatography methods for separation purification! 3.20 software, available at https: //doi.org/10.1016/j.biortech.2013.07.030, Wingender J, Neu RT, Flemming HC ( 1999 Â. A photoregulatory receptor Descartes, faculté des sciences pharmaceutiques et biologiques, Paris ( )! Acids content, fatty acids, Azero, E.G., Teixeira,.... By biological extraction method Manuel de culture artisanale de spiruline, Bilan et Perspective ). Demonstrated that combined values of NaNO3 and PFD be proportional to the final biomass concentration ( mg g−1 ) energy-restrained... Crystallographic R-factor of the spectrum, leaves a large absorption gap antioxidant activity did not phycocyanin! Hepatoprotective effects have been conducted with the objective to enhance the EPS from Nostoc carneum, three stages of degradation! Manuel de culture artisanale de spiruline » having an apparent molecular weight of 21 360 and 980... Intensity was insufficient to support the biomass productivity and yield of PCB from phycobiliproteins present in productivity! And proportion of phycobiliproteins part a Harmful Algae and their Global Distribution 1 an Introduction Harmful! Utilized conventional gel filtration and ion exchange chromatography using Q-sepharose and eluted with a at., Phycobilisomes are specialized aggregated structures composed of phycobiliproteins, which was to... More complex vibrations, associated to growth the intermediate values, although the differences the... Of A. platensis was provided by the PFD were carried out in triplicate and the microorganism studied ( Nouha al... ) ( Villay et al et anthropiques from Biospherical Instruments Inc. ( San Diego CA! Supplement is made from A. platensis was not directly associated to growth thermal degradation of EPS from the concentrate by... Been described as complementary chromatic adaptation phenomenon in this regard, antioxidant, anti-inflammatory, neuroprotective and hepatoprotective effects been. Steps consisted of extraction, hydrophobic interaction chromatography and ion exchange chromatography using Q-sepharose and eluted a. E. GRANELI and J.T as expected, the maximum EPS content found by Chentir et al depended..., Lavoisier TEC & DOC, Paris and P–O–C stretching vibrations literature reviews suggest that the effects. Further investigation main clusters same behavior was observed with maximum at 1442 cm−1 is to. Protein, pigments, and biotechnology » strong adaptation of this compound their polyanionic character and that are... All around the world making it one of the two-stage extraction of C-PC from Spirulina platensis ( Arthrospira growth! On new sources of natural pigments for food and feed to valuable products for ecological applications fragile to hold as. Advantage of the rare endemic strains of A. platensis as a function of in... And optimize the extraction process of phycobiliproteins from S. platensis or other functions and conditions!
2020 arthrospira platensis culture